J Tissue Eng Regen Med. 2017 Jul 17. doi: 10.1002/term.2514. [Epub ahead of print]
题目:
Role of the protease corin in chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells.
作者:
Zhou H1,2, Zhu J1,2, Liu M1, Wu Q1,3,4,5, Dong N1,3,5,6.
单位:
1Cyrus Tang Hematology Center, Soochow University, Suzhou, China.
2Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, China.
3Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.
4Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA.
5Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Soochow University, Suzhou, China.
6Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Suzhou, China.
摘要:
Mesenchymal stem cells (MSCs) have the potency to differentiate into chondrocytes, osteocytes and adipocytes. Corin is a cardiac protease that activates the natriuretic peptides, thereby regulating blood volume and pressure. In addition to the heart, corin gene up-regulation was reported in bone marrow- and adipose tissue-derived MSCs that underwent osteogenic differentiation. To date, the biological significance of corin expression in MSC differentiation remains unknown. In this study, we isolated and cultured human bone marrow-derived MSCs that were capable of undergoing chondrogenic, osteogenic and adipogenic lineage differentiation. By RT-PCR and immunostaining, we found that corin expression was up-regulated when these MSCs underwent chondrogenic, osteogenic and adipogenic differentiation. The up-regulation of corin expression was most significant in the cells undergoing chondrogenic lineage differentiation. Silencing of corin gene expression by small hairpin RNA in the MSCs inhibited chondrogenic, but not osteogenic and adipogenic, differentiation. These results suggest a novel function of corin in MSC differentiation and chondrocyte development.